Thursday, March 15, 2012

Baltimore police search for missing baby, sitter

BALTIMORE (AP) — A 7-month-old boy and a teenager who was left to briefly watch him have not been seen for days and the baby's mother on Sunday pleaded for her son's safe return.

Ki'Yauhn Birch was left in Jonae Boozer's care at the boy's father's home in Baltimore on Friday evening.

During a support rally Sunday night, the baby's 20-year-old mother, Whitney McGee, tearfully pleaded, "Put my baby somewhere safe and call somebody. He's probably hungry."

The baby's father, Charles Birch, arrived late, saying he had been questioned by police.

Birch said he barely knows Boozer, who is a friend of a friend. The girl stopped by his home on the west side of Baltimore and …

Stocks falter after drop in pending home sales

Stocks clung to a tight range Thursday after an unexpected drop in pending home sales raised new concern about the economy.

Major indexes were little changed in afternoon trading following the National Association of Realtors' report that its index of home sales agreements fell 7.6 percent in January from December. Contracts fell to the lowest level since April.

The drop in the number of buyers of previously occupied homes dampened some of the enthusiasm about better retail sales numbers. Macy's Inc., Limited Brands Inc. and The Wet Seal Inc. all posted monthly sales that topped analysts' expectations. Wal-Mart Stores Inc. raised its dividend 11 …

Sex through the ages

Sex being the simplest route to increased TV ratings, it's a smallwonder that even the History Channel has worked around this season toa new presentation entirely devoted to the subject.

"Sex in the 20th Century," which began Monday and continues from 9to 10 each night through Thursday, offers a carnal chronology ofmodern times, arguing that "human sexual relations have changed morein the past 100 years than in the previous 10,000."

That's debatable. Since the days of biblical lore and Greek myth,the usual biological drives-hitched to the specific furies of thetime-have been a mighty force for experiment, conflict and,sometimes, downright havoc.

From such …

Wednesday, March 14, 2012

Rockies lose 10-7 to Diamondbacks

DENVER (AP) — Center fielder Dexter Fowler is establishing himself as a key contributor as the season winds down for the Colorado Rockies. Pitcher Esmil Rogers is still trying to find his way.

Fowler homered from both sides of the plate Monday, but Rogers was hit hard in Colorado's 10-7 loss to the NL West-leading Arizona Diamondbacks.

The Rockies have lost six straight against Arizona, which improved to 12-4 in the season series against the division rival.

Fowler continued his surge since being recalled from Triple-A Colorado Springs on July 15. He has four homers in six games after going 382 at-bats without a home run before his first this season on Aug. 30.

The …

Iraq: 2 bombs hit Baghdad, killing 1, wounding 7

Two bombs apparently targeting Iraqi security forces struck different areas in Baghdad on Tuesday, killing at least one civilian and wounding seven others, Iraqi officials said.

The first explosion occurred just before 9 a.m. in northern Baghdad as a police patrol passed through the Sulaikh area, but it missed its target and hit a civilian car instead, wounding four people, police said.

The U.S. military said the blast was caused by a pipe bomb and three children were wounded, two seriously. The attack was reported by the U.S.-allied Sunni group in the area, it said.

Another …

Care home whistleblowers are rewarded by police for bravery

Two care assistants whose suspicions led to one of the biggestpolice investigations in central Somerset were rewarded for theirbravery by police on Tuesday.

Sarah Barnett and Kathleen Slade were both given the Waley CohenAward, presented to members of the public who perform acts ofbravery in support of law and order.

While working as care assistants in at Parkfields ResidentialCare Home in Butleigh, Sarah Barnett and Kathleen Slade becamesuspicious of the activities of manager Rachel Baker fromGlastonbury, who they believed was fabricating symptoms of theresidents in order to obtain prescribed medication for her ownpersonal use.

As a result of …

Man Gets 18 Months in '84 Attack

CHARLOTTESVILLE, Va. - A man who sexually assaulted a University of Virginia student in 1984 and apologized to her two decades later as part of the Alcoholics Anonymous program was sentenced to 18 months in prison Thursday.

William Beebe, 42, pleaded guilty in November to one count of aggravated sexual battery for his attack on Liz Seccuro.

Charlottesville Circuit Court Judge Edward Hogshire ordered a 10-year prison sentence with all but 18 months suspended, as long as Beebe performs 500 hours of community service related to issues of sexual assault and alcohol abuse on college campuses. Prosecutors had recommended two years.

"I'm not trying to excuse my …

US stocks rise on gain in services sector

Wall Street is extending a moderate advance following a better-than-expected reading on the service sector.

Stocks are rising following the Institute for Supply Management's report that the service sector contracted at a slower pace in January than …

Ten great getaways for a long weekend

Travel-industry experts say that more and more of us are takinga number of short getaway trips each year rather than one longvacation. And summer is prime time for these weekend excursions frommetropolitan Chicago to a variety of tempting Midwest destinations.

There are dozens of possible summer-weekend jaunts, dependingon your tastes and budget. To help start your motor, here's apersonal selection of 10 top quick-trip targets. All within 200miles of Chicago, they range from the obvious perennials to a placeor two you may never have considered.

Because so many folks hit the road in summer, it's wise to makeroom reservations as far ahead as possible. That's …

Sunni and Shiite Iraqi journalists talk about war

BAGHDAD (AP) — As the U.S. draws down in Iraq, two Associated Press Television News cameramen, one Sunni and one Shiite, talk about what it has been like to live through and record the war. The accounts are translated and edited.

Faris al-Qaisi, 47, is a Sunni with two daughters and a son. He served in the Iraqi military for nine years and fought during the 8-year war with Iran. He was working for a Lebanese steel company before the U.S.-led invasion of Iraq in 2003. He joined Associated Press Television News two months after the fall of Baghdad.

__

I did not expect the country would fall so easily.

I suffered during the former regime of Saddam Hussein because I …

Total UK admits charges in huge 2005 oil fire

Oil company Total UK pleaded guilty Friday to three charges stemming from a massive oil depot fire in 2005, the biggest conflagration in Europe since World War II.

At a hearing in the Central Criminal Court in London, Total submitted written guilty pleas to two charges under the Health and Safety Act and one of polluting water stemming from the explosion and fire at the Buncefield oil depot north of London on Dec. 11, 2005.

Forty-three people were injured in the incident and 2,000 people living nearby were evacuated, but no one was killed.

Four other companies pleaded not guilty to health and safety violations. They are Hertfordshire Oil Storage …

Men sentenced after attacking pub doorman

Three men who acted like "drunken teenage louts" when they beatup staff at a Keynsham pub have been given suspended prisonsentences.

Drunken Julian Connor, Paul Hervin and Andrew Shorney brawledwith the bar manager and doorman at The Pioneer pub.

The fracas began after depressed Connor took an overdose and thenwent drinking with his pals.

Once at the pub they attacked the doorman for no reason.

Connor, 34, of Mendip Close, Keynsham, Hervin, 39, of Downfield,Staple Hill Grove, Keynsham, and Shorney, 31, of Coronation Avenue,Keynsham, were caught on CCTV.

At Bristol Crown Court, all three admitted affray and receivedthree-month prison sentences, suspended for 12 months. They mustalso pay their victims pounds200 compensation.

Connor also received a 12-month supervision requirement and hisco-defendants were given 150 hours of unpaid community work.

Paul Grumbar, prosecuting, told the court that doorman VitterioBecker and bar manager James McGregor were injured in the affray onApril 28 last year.

He said the trio had been rowdy when they arrived.

"Shortly afterwards there was an unprovoked attack. Connor wentover to Mr Becker and threw a punch at his face. Mr Becker foughtback," said Mr Grumbar.

He said Hervin hit Mr Becker on the head with a glass and thensmashed a pool cue across his back.

When Mr McGregor went to help the doorman, Shorney attacked him,knocking to the ground and kicking him.

The three ran out but thanks to CCTV images, they were laterarrested.

Mr Becker, who is also a roofer, suffered a badly bruised backand internal damage, as well as cuts to his head, and was off workfor a week.

Mr McGregor suffered bruised and swollen arms in the attack.

Nicholas O'Brien, defending Connor, said his client had a goodjob with a firm of metal fabricators before becoming depressed in2006.

He said the defendant had a row with his partner on April 28 andhad taken an overdose of anti-depressants before going to the pub.

He said Connor was now motivated to seek help and tackle hisdrinking.

Ramin Pakrooh, defending father-of-two Hervin, who had also beensuffering from depression, said his client had not intended to usethe glass as a weapon.

David Chidgey, defending delivery man Shorney, said his clientaccepted Mr Becker was doing his job when he attacked him, and wasremorseful.

Recorder Mr Paul Derbyshire told the trio: "Although all three ofyou are in your 30s, you acted like drunken teenage louts in ThePioneer that evening. You terrified the bar staff."

Waiting for Jackson action

WASHINGTON The mantra "Run, Jesse, Run" has started up again.

While the Rev. Jesse L. Jackson, a two-time presidentialcontender, has made it clear he wants to be a player in the nextWhite House contest, he hasn't decided just how.

The topic came up at a recent Rainbow/PUSH Coalition conferencein Chicago after Jackson's family and supporters urged him to make adecision to lay groundwork for a presidential run in 2000.At the meeting, Jackson, president and founder of theorganization, was coy when a woman got up and read a poem on thetheme "Run, Jesse, Run." He praised her work for its "aesthetic"value.Heavier pressure came from his son, Rep. Jesse Jackson Jr.(D-Ill.), who told the Chicago Sun-Times that he thought his fatherwould run and pressed him to get started now. Longtime backers atthe conference also lobbied him.Through all the nudging, Jackson remained a sphinx.Can the 55-year-old minister stage an electoral comeback afterskipping two presidential contests? Supporters acknowledgeskepticism, but operatives in other camps insist Jackson still hasthe magic.Even Vice President Al Gore, the apparent front-runner, iskeeping an eye on the competitor who outlasted him in the 1988primary. Encountering the elder Jackson in the White House lastweek, the vice president joked about his recent publicity."Tell Congressman Jackson before he encourages you to run, weneed to have a meeting of the St. Alban's alumni association," Gorejoshed, according to Jesse Jackson Sr. Jesse Jackson Jr. and Goreattended St. Alban's School.Support from African-American voters would give Rev. Jacksonpunch in 2000. He built his historic showing in the 1984 and 1988elections on the base of black votes, and key figures in otherpolitical camps say that he could do it again.Three days after the Chicago meeting, Jackson talked at lengthabout the situation."I have all the credentials to run," he said. "Whether I run ornot will be determined on factors not yet to be discussed. But Iintend to be a factor in determining the course of our party and ourcountry."He wants a debate over what the Democratic Party should standfor. He fears the party is shifting too far to the center and is notpaying attention to the needs of the poor."A vast body of Democrats feel they are not in the debate," hesaid. "The middle class is anxious and afraid, and the poor aredesperate."It's up to me to decide not to run. I have the know-how,experience, public acceptance, track record for service andrecognition based on service has been established."Then why not send a definitive signal?Supporters are frustrated he hasn't been clear about his plans.Other candidates already are lining up workers, money and othercommitments that Jackson could compete for.In Illinois, allies of Gore and House Minority Leader RichardGephardt (D-Mo.), another top contender, are starting to gathersupport, sources said. One Gephardt supporter even worked theRainbow/PUSH conference, a source said."I'm studying all my options," Jackson said.Is running for president among them?"Of course," he replied.Why not decide now?"Because it's weighty, I haven't made it yet," he said.Others pressing Jackson include Frank Watkins, press secretaryfor Jackson's 1984 presidential bid and political director of his1988 effort. Watkins, now working for Rep. Jackson, is frustratedabout the slow start."Every indication I've seen is that he's inclined to run," saidWatkins, but he should take steps now to "at least freeze his people.There are ways of making intentions known without an announcement."Rep. Maxine Waters (D-Calif.) and Boston political activistMel King Jr., who worked in Jackson's presidential campaigns, stokedthe fires in Chicago. Waters said he must decide this year, and Kingasked him to decide now."Jackson is the only progressive voice who is presidentialmaterial, has support all over the country and a base, and could be acredible spokesperson on behalf of many of us," Waters said.David Bositis, of the Joint Center for Political and EconomicStudies, said that only Jackson can stop Gore from locking up thenomination in Southern states on Super Tuesday - just as Clinton didin 1992.Bositis' thesis is that Jackson can keep close to Gore in theSouth, where blacks remained half the Democratic vote after whitesleft the party. Wounded in the South, the more moderate Gore wouldhave to face more liberal working-class issues in later states,including Illinois. Challengers including Jackson, Gephardt and Sen.Paul Wellstone (D-Minn.), who is looking at running, could force Goreto debate and embrace the more liberal issues - shifting the partymore to the left.Jackson's strength was reflected in Bositis' nationwide poll of850 blacks and 728 whites taken last spring. Blacks were 86.6percent favorable to Jackson and 8.1 percent unfavorable. Whiteswere 29.9 percent favorable, 50 percent unfavorable.Because of Jackson's southern potential, Bill Carrick, theCalifornian who ran Gephardt's 1988 presidential bid, and the Rev.James Wall, who was chairman of Gore's 1988 Illinois campaign,predict Jackson will enter the race."If he runs or gets behind some other candidate, he could bedeterminative of the nomination," said Carrick, noting that one-fifthof the delegates to the next Democratic convention are expected to beblack."I'm assuming Jackson is going to be a candidate because hewants to see his issues raised," said Wall, who ran PresidentCarter's Illinois campaigns. "Gore is going to have to deal with thereverend."

Tuesday, March 13, 2012

Soaring energy, food push inflation up at fastest rate in 27 years; weak retail sales

The economy showed the depth of its twin problems on Tuesday, slow growth and rising inflation, as the nation wrestled with a teetering financial system, a slumping dollar and rising prices for food and fuel.

The Labor Department reported that soaring costs for gasoline and food pushed inflation at the wholesale level up by a bigger-than-expected 1.8 percent in June, leaving inflation rising over the past year at the fastest pace in more than a quarter-century.

Over the past 12 months, wholesale prices are up 9.2 percent, the largest year-over-year surge since June 1981, another period when soaring energy costs were giving the country inflation pains.

Core inflation, which excludes energy and food, was better behaved in June, rising just 0.2 percent, slightly lower than expectations.

A separate report from the Commerce Department showed that all the economy's problems were weighing on the consumer.

Retail sales edged up by a tiny 0.1 percent in June, weaker than had been expected by Wall Street analysts, as consumer spending was held back by a sharp plunge in sales at auto dealerships.

In midmorning trading, the Dow Jones industrial average dropped 209.57, or 1.90 percent, to 10,845.62. Broader U.S. markets also were down.

Banking stocks were pounded on Monday despite the government's efforts to calm concerns with a support package fashioned over the weekend for mortgage giants Fannie Mae and Freddie Mac.

Federal Reserve Chairman Ben Bernanke said Tuesday that the fragile economy was being confronted by "numerous difficulties" including persistent strains in financial markets, rising joblessness and housing problems. He said rising prices for energy and food were elevating the risks of inflation.

Delivering his midyear economic report to the Congress, Bernanke said the current situation poses "significant challenges" for Fed policymakers as they try to chart the best course for keeping the economy growing, while making sure inflation doesn't dangerously flare up.

The Fed signaled an end to an aggressive rate-cutting campaign in June because of growing concerns about inflation. Bernanke kept up his tough anti-inflation talk on Tuesday but stressed many other problems that could short circuit economic growth.

In a third economic report, the Commerce Department said business inventories rose at a slower-than-expected pace in May, a possible indication that the weakening economy is making companies cautious on their restocking plans.

The department said inventories held on shelves and backlots edged up 0.3 percent in May, smaller than the 0.5 percent gain that many economists had been expecting. It was the smallest monthly increase since inventories had risen just 0.2 percent in April.

For June, energy prices at the wholesale level shot up by 6 percent; the price of unleaded regular gasoline surged by 9 percent following an even bigger 9.6 percent increase in May.

The 0.1 percent rise in retail sales was even weaker than the 0.4 percent gain that analysts had been expecting.

That small rise reflected a 3.3 percent drop in sales at auto dealerships, offsetting a big 4.6 percent jump in sales at gasoline stations, an increase that largely mirrored last month's huge jump in pump prices.

General Motors said Tuesday that it plans to lay off salaried workers, cut truck production and suspend its stock dividend, showing the depth of the U.S. auto industry's mounting troubles as it adjusts to a declining U.S. market.

GM said it would also borrow $2 billion to $3 billion as part of an effort to raise $15 billion to help turn around its North American operations.

North Carolina jobless claims overwhelm Web site

Unemployment is up so much in North Carolina that the state's Internet site for benefits crashed twice this week under a rush of claims.

Once the system was back up, the state set one-day records both for the amount of unemployment benefits paid and for the number of transactions, officials said Tuesday.

The number of people trying to sign up online for new or continuing benefits was as much as triple pre-recession levels Sunday and Monday, the Employment Security Commission said. That volume, together with a phone line problem, overwhelmed the agency's computers and prevented some people from filing claims.

The system was working again by Monday afternoon after the ESC added another server and demand lessened, said ESC spokesman Andy James.

On Monday, the state paid more than $31.5 million in unemployment insurance benefits and handled more than 106,000 transactions _ both one-day records, James said. The previous records weren't immediately available, but James said the Web site might have gotten 23,000 hits on a busy day 18 months ago.

The phone line problem was fixed Sunday, when 74,000 people tried to access the system, and the ESC thought the overload problem had been handled as well. But the system failed again Monday when about 55,000 people tried to file.

Mark Turner, who was laid off in November as a company support manager but has since landed a six-month contract job, said he spent hours Sunday night trying to access the system unsuccessfully.

"It's a bad sign for North Carolina's economy when you've got so many people trying to get into the system and it crashes," he said.

The recession has pushed the jobless rate in North Carolina to 7.9 percent in November, the last month for which figures are available. That was the highest since October 1983.

The ESC said payment will be delayed about a day to people who couldn't file Sunday. People who couldn't access the system Monday had until 9 p.m. Monday to get their payment the same day they normally would.

North Carolina's ESC has about 300,000 people in its unemployment system and pays about $33 million a week in benefits.

Bloomingdale Man Accused of Nazi Role

WASHINGTON A Bloomingdale, Ill., man is accused of having workedfor the Nazis at the notorious Auschwitz and Buchenwald Lauraconcentration camps, guarding inmates as they were evacuated fromcattle cars and handling the vicious dogs that kept them in line.

"These guard dogs were trained by their handlers to viciouslyattack concentration camp inmates on command and to bite or maulinmates who violated arbitrary rules relating to inmate discipline,"a U.S. Justice Department statement said Friday.

Justice officials say Wiatscheslaw "Chester" Rydlinskis, wholives in Bloomingdale's Medinah Lakes subdivision, concealed fromU.S. immigration authorities his activity during World War II as aconcentration camp guard and a guard dog handler.

In making the charges Friday, the Office of SpecialInvestigations, which hunts down former Nazis in the United States,asked a federal judge in Chicago to strip Rydlinskis of his U.S.citizenship.

The U.S. government said Rydlinskis, who was born in Lithuaniain 1924, joined the Waffen SS in 1941 and served from 1941 to 1945 inthe Death's Head Battalion at Auschwitz, the concentration camp inNazi-occupied Poland, and at the Buchenwald Laura subcamp in Germany.

At the German camp, he allegedly guarded inmates as they wereevacuated from cattle cars and were forced to march to the Dachauconcentration camp.

Rydlinskis allegedly was transferred later to a guard dogcompany at Auschwitz.

He was arrested in 1945 by British military forces. He wasdetained as a war-crimes suspect for almost two years, but he was notprosecuted and was released on May 28, 1945.

He obtained a visa to emigrate from Germany to the UnitedStates in 1956 by concealing his wartime background, the JusticeDepartment charged.

The government alleged that Rydlinskis hid his past again in1966 when he was naturalized as a U.S. citizen.

Tony Cozzi, block captain for a neighborhood watch program,said Rydlinskis and his wife have lived in the town house complex forabout 1 1/2 years.

"He seemed to be very nice, a very mild-mannered person. Mostnaturally I'm surprised," Cozzi said. "Who would ever think?"

Neighbors Robert and Metta Clancy said their contact with himwas limited to an occasional greeting on the street.

"He seems like a very nice, ordinary guy," Metta Clancy said.

The Justice Department unit that tracks down Nazis has persuadedcourts to rescind the citizenship of 50 former German soldiers and todeport 42 of them from the country. "The bulk are from Chicago andCleveland," a spokesman said.

Contributing: Alex Rodriguez

Germany, Greece sign agreement to boost investment

VOULIAGMENI, Greece (AP) — Germany and Greece signed an agreement on Friday aimed at boosting investment in the debt-drowned country and getting its economy growing again.

German Economy Minister Philipp Roesler and Greek Development Minister Michalis Chrisohoidis said the agreement was part of a strategy to help Greece deal with its crisis by helping its real economy rebound.

"We believe that Greece can become a very important investment destination," said Roesler, who is on a two-day visit to Greece with a delegation of dozens of German businessmen. "We're sending out the message that we can implement those things that we have decided on."

Roesler said deals worth €2.5 million ($3.36 million) have already been made creating 50 new jobs in Greece, adding that there was additional German interest in investing in major infrastructure projects in the country worth €1.5 billion, including pipeline construction.

"I believe the energy sector is one that is truly geared towards the future for Greece," Roesler said.

The German official said the investment agreement sets a legal framework and clears administrative hurdles that aim to impart a "sense of security" to businessmen seeking to invest in the country. He said German Development Bank KfW is ready to lend its expertise for the creation of a similar development bank in Greece to support small and mid-size businesses.

Chrisohoidis said a new program will be unveiled next month that would allow small and mid-size businesses more access to funding, while the European Union is working on amending regulations making it easier for Greece to fund large infrastructure projects and other businesses.

The agreement also includes a new financing tool through KfW's "Entrepreneur Loan" program offering official backing to German companies wishing to invest in Greece.

Germany would also help Greece absorb EU support funds geared toward bolstering the Greek economy's competiveness.

"We have to see this as a very positive, very strong message to the markets," said Roesler.

Roesler said he would convey the message to other EU partners that Greece offers good investment opportunities and that an investment in Greece would translate into an investment in Europe. Chrisohoidis said he would travel to the French capital next week to encourage such investment in his country.

Greece's economy is projected to shrink by 5.5 percent this year as the country struggles to meet austerity targets set as a condition for it to continue receiving funds from a €110 billion ($145 billion) package of international bailout loans that are preventing it from defaulting on its debts.

Finance Minister Evangelos Venizelos has said that Greece has enough money to pay pensions, salaries and bondholders through mid-November. But the country needs the next batch of loans, worth €8 billion ($10.5 billion), to avoid bankruptcy.

Most investors expect an eventual Greek default which would send shockwaves throughout the slowing world economy, stunting recovery and prolonging recession.

Debt inspectors from the International Monetary Fund, European Central Bank and European Commission, collectively known as the troika, are now in Athens evaluating reforms before the funds are released.

Jean-Claude Juncker, Luxembourg's prime minister who also heads the group of eurozone finance ministers, said he expects the troika evaluation to be ready by Oct. 24.

Speaking after talks with Roesler earlier Friday, Venizelos said eurozone price and currency stability is crucial to generating growth. He said Greeks must be convinced that the deep salary cuts and tax hikes they are enduring will produce results while German taxpayers must be convinced that their assistance will benefit all of Europe in the long run.

"And that is the case because Greece will honor this assistance up to the last euro," Venizelos said.

Roesler acknowledged that Greeks are sacrificing much and that strong public reaction to austerity measures is understandable, but said it shouldn't detract from the goal of implementing reforms. He praised the Greek government for having the will to push through those reforms.

(This version CORRECTS Corrects that deals worth 2.5 million euros instead of 2.5 billion made in paragraph 4.)

Rybak sparks Texas State to 97-92 overtime victory

John Rybak came up with two key steals and six of his eight points in the overtime period Saturday, helping Texas State defeat Lamar 97-92.

The back-to-back steals led to three points for Texas State (9-10, 3-2 Southland), putting the Bobcats up 92-87 with 1:16 left in overtime. After Lamar (10-10, 2-3) cut it to 92-90 on a layup by Kendrick Harris with 17 seconds remaining, Rybak hit four straight free throws to seal the victory.

Cameron Johnson led six Texas State players in double figures with 24 points and 15 rebounds. He hit 1 of 2 free throws with 7 seconds remaining in regulation to send the game to overtime.

John Bowman added 15 points for the Bobcats, while A'Moute Bidias had 10 points and 17 rebounds.

Harris had 22 points and Anthony Miles 21 for Lamar. Justin Nabors added 18 points and 10 rebounds, and Lawrence Nwevo had 15 points and 12 rebounds.

Airline to Test Anti-Missile System

Up to three American Airlines jets will be outfitted this spring with laser technology being developed to protect planes from missiles fired by terrorists.

Officials said Friday the anti-missile systems won't be tested on passenger flights. But the tests, which could involve more than 1,000 flights, will determine how well the technology holds up under the rigors of flight, they said.

The first Boeing 767-200 will be equipped in April or later, American spokesman Tim Wagner said. American operates that Boeing model mostly between New York and San Francisco and Los Angeles.

American said it is "not in favor" of putting anti-missile systems on commercial planes but agreed to take part in the tests to understand technologies that might be available in the future.

The anti-missile technology was developed for military planes, and U.K.-based BAE Systems PLC said Friday it won a $29 million contract from the Department of Homeland Security to test it on passenger planes.

The technology is intended to stop a missile attack by detecting heat from the rocket, then responding in a fraction of a second by firing a laser beam that jams the missile's guidance system.

The device on the belly of the Boeing 767-200 aircraft will be operational but won't be tested on regular flights, Wagner said. The use of a signal to mimic a missile attack has already been tested in the air, Wagner said.

Those tests also showed that the anti-missile systems didn't interfere with the jet's other controls, officials said.

American, the nation's largest carrier, has been working with BAE on the project for a couple years. In 2006, BAE installed its hardware on a Boeing 767 that wasn't used to fly paying passengers.

About a year ago, BAE invited reporters to American's maintenance base in Fort Worth to see a jet outfitted with the laser-jamming device called Jeteye.

"We are now entering the next phase," Wagner said, which is "to see how the system holds up on an aircraft in real-time conditions _ weather, continuous takeoffs and landings, etc. _ and to test its maintenance reliability."

Burt Keirstead, director of BAE's commercial airline protection program, said BAE's contract requires it to prove that Jeteye will operate without failure for 3,000 hours of flight and sets a goal of 4,500 hours.

"If there is one aspect of performance that is hardest to satisfy, it's reliability," Keirstead said. "We predict we'll meet the (3,000-hour) threshold, and we hope to get to the (4,500-hour) goal."

BAE expects to test the device through 7,000 hours of flying in 2008 and early 2009, he said.

With the latest contract, BAE has received more than $100 million in funding for aircraft-protection systems. Keirstead said BAE's technology will cost $500,000 to $1 million per plane to install.

Congress has approved funding for anti-missile research partly out of fear that terrorists armed with shoulder-fired weapons could hit jetliners as they take off and land.

Fort Worth-based American, a unit of AMR Corp., has said anti-missile defense is best handled by stopping terrorists from getting missiles that could shoot down commercial jets and by improving security around airports.

Keane's return live on Setanta

Setanta Sports will begin their coverage of the Barclays PremierLeague with Liverpool's trip to Aston Villa on the opening day of theseason, August 11.

Newcastle's clash with Aston Villa will follow on Saturday 18 withEverton against Blackburn a week after.

Roy Keane's return to Manchester United as manager of Sunderlandwill be shown on September 1.

Newly-promoted Derby's encounter with Newcastle will be Setanta'sfirst Monday night fixture on September 17.

Monday, March 12, 2012

Biosolids use increases pine production

This case study in a pine plantation shows how biosolids significantly improved site productivity without adverse effects on the ecosystem.

APPROXIMATELY 77,000 dry tons of municipal biosolids are produced in New Zealand every year. Traditionally, most solid residuals from wastewater treatment plants are disposed of by landfilling. In recent years, however, land application is becoming a popular option for managing municipal biosolids, due to increasing public, cultural and regulatory pressures to protect the environment. The New Zealand Waste Strategy 2002 suggests that more than 95 percent of municipal biosolids are expected to be beneficially used by December 2007. This policy has enhanced the significance of applying municipal residuals on to land. Application to forested land, in particular, can significantly minimize the chances of contaminants entering the human food chain.

Since the mid-1990s, biosolids have been applied to a nearby 1,000 ha of pine forest plantation growing on a sandy, low fertility soil at Rabbit Island in Nelson, New Zealand. This was the first full-scale biosolids land application operation in New Zealand. The biosolids were aerobically digested liquid (one to three percent solids) and applied using a specially built spreader. The biosolids contained high concentrations of nitrogen (eight to ten percent N) and were low in heavy metals. To investigate the effects of biosolids application on tree growth, nutrition, soil and ground water quality, an experimental research trial was established at the island. Biosolids were applied in 1997 and 2000, at three application rates based on the nitrogen loadings: O (control), 300 (standard, operational rate) and 600 kg N ha^sup -1^ (high), to pine trees planted in 1991.

Biosolids treatment has significantly increased tree growth by increasing tree diameter (and basal area) and volume increment. In August 2002, at age 11, the average tree basal area of the high treatment was 48 percent and the standard treatment was 30 percent greater than the untreated control treatment. Tree volume of the high treatment was 52 percent and the standard treatment was 34 percent greater than the control treatment. These large increases of wood volume in biosolids treatments do not appear to have reduced basic wood density. The significant increase of basal area and live volume of the trees in biosolids treatments were mainly attributed to improved nitrogen supply. Nitrogen concentrations in pine foliage sampled in the summer of 2002 were 1.10 percent, 1.34 percent and 1.49 percent N for the control, standard and high treatments, respectively.

Natural abundance of stable N isotope ^sup 15^N ([delta]^sup 15^N) can potentially be used to identify the source of N in a given ecosystem. Biological treatment of sewage and digestion of sewage sludge resulted in the discrimination against the heavier isotope of N, resulting in the enrichment of ^sup 15^N in the biosolids. For example, [delta]^sup 15^N values in the Nelson biosolids were between 5.00 and 8.71[per thousand]. In contrast, N-limited coniferous forest soils are generally ^sup 15^N depleted and this was reflected in [delta]^sup 15^N in foliage of the control treatment ([delta]^sup 15^N - 1.45 percent). The elevated [delta]^sup 15^N in biosolids treated pine foliage ([delta]^sup 15^N 1.07 percent in the standard and 5.51 percent in in the high treatment) indicated that a considerable amount N was sourced from biosolids. Analysis of [delta]^sup 15^N in representative weeds indicated that both non-legumes and legumes took up N from the biosolids and acted as an N sink, reducing N availability for leaching.

Soil analysis in 2001 indicated that biosolids application did not have any significant effects on many soil chemical properties, for example, no significant changes in soil acidity and concentrations of total organic carbon, nitrogen, heavy metals and exchangeable calcium, magnesium and potassium were observed. The high rate biosolids treatment increased the sodium bicarbonate extractable phosphorus in the soil. Groundwater quality, which is monitored quarterly at the trial site, showed that the concentrations of NO^sub 3^- and heavy metals were well below the Maximum Acceptable Values for inorganic substances of health significance defined by the New Zealand Drinking Water Standard.

In summary, application of biosolids to the pine plantation at Rabbit Island has significantly improved the site productivity and has not caused any measurable adverse effect on the receiving ecosystem.

[Author Affiliation]

Hailong Wang and Gujja N Magesan are environmental soil scientists specializing in beneficial utilization of municipal and industrial wastes, at Forest Research, Rotorua, New Zealand (hailong.wang@forestresearch.co.nz). This research has been funded by PF Olsen Ltd and New Zealand Foundation for Research Science and Technology. The authors are grateful to Peter Wilks, Craig Fisher and Mark Kimberley for their input in this project.

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Who says tastes change? The Fig Newton hits 100

What is "ooey, gooey, rich and chewy inside; tender, flaky,golden cakey outside" and is 100 years old? Give up? It's the FigNewton. On May 7, Nabisco Brands will celebrate its cookie'sbirthday by donating 25 fig trees to the town of Newton, Mass., forwhich the popular cookie was named. And who says tastes change?Sixty-five million pounds of Fig Newtons are consumed annually. Whoelse would tell you these things?

BIRD BITS: That bird that continually crashes against your largepicture window isn't trying to build a nest in your home. Heprobably sees his reflection in the glass and presumes that it is arival flying into his territory. This is the opinion of JamesFizzell, a University of Illinois horticulturist. Here is Fizzell'shelpful hint: "Break up the reflection in the window by pullingdrapes over the window or hanging up a sheet. The simple way toreduce the reflection is to place an `X' of tape over the window toconvince the bird that the reflection is not a rival trying to stealhis mate."

GOURMET TIDBITS: To obtain the most nutrients from freshproduce, shop for them frequently and buy only as much as you willuse in a few days. If your produce sits in the fridge for a week,it's better to opt for frozen fruits and vegetables. They willretain more vitamin C than fresh vegetables kept too long.

BEAUTY BRIEFS: If you need the strength of mousse to set yourhair, but find that it is drying, use half mousse and half settinglotion. When there is no time for a shampoo, pour witch hazel into asmall bowl. Dip your fingertips into the mild astringent and massageit into your scalp. It will not disturb your set, and will freshenyour scalp until you have time for a shampoo.

TIMELY TIPS: A firm, well-padded ironing board is a must to makeyour ironing quicker and better. If your old board wiggles orwobbles, it's high time you got a new one. Also buy a plastic sheetto cover the floor area before you iron sheets or tablecloths. Whenyou dampen clothes, use warm water. Iron low-heat fabrics first, andthen go on to cotton and linen temperatures. (This preventsscorching.) Stop guessing how much water to use to fill your steamiron. Mark your glass measuring cup with red nail polish to show howmuch you need.

A dampened cloth dipped into baking soda will clean the bottomof an iron. If the soleplate is very sticky, pour salt onto a pieceof brown paper. Run the iron over the salt. Then "iron" a piece ofwaxed paper. Your iron will glide smoothly!

DEAR DORSEY: We read so much about water shortages all over thecountry that we should all resort to ways to save water. For years Ihave collected rain water. I have large plastic buckets that I placeat strategic places where the water runs off my roof. I then fillplastic bottles with the water and store them in the garage. Ialways have enough water when it doesn't rain to water my plants andthe flowers in my garden. Saved rainwater is also great for cleaninghands that have been working in the garden (the water is so soft youneed little soap). And I use it for washing garden tools, dust mopsand dust rags. It sure saves on the water bill!

CAROLE KUBIK

Thank you, Carole, for presenting a very important idea. Weshould all be more conscious of saving water. Any more ideas outthere in readerland?

DEAR DORSEY: When my nephew was a teenager looking forneighborhood jobs, his business-smart father had inexpensive businesscards printed for his son. On these cards along with his name andtelephone number, were listed the kinds of services that his son wasable and prepared to do. This garnered a lot of responses and mynephew was a busy lad for some time thereafter.

MASON SEGLIN

For some, repeat of flooding tests their faith

The Furey brothers figure someone upstairs must be testing them.

As John Furey sat on an island in the flooded kitchen of his brother Pat's home in Pearlington Tuesday, he pondered this latest forced exit from home.

Pearlington was practically wiped off the map by Katrina. Since 1964, John's home had flooded only twice, during Katrina and now Gustav.

"The Lord must be testing our faith," said John, 65. "I don't know what I did. He's just testing me."

His 70-year-old brother, Pat, shook his head and smiled at the thought.

"That's right. He's just testing us."

As the two ate in the damp, powerless home, John said locals are upset. He just finished settling with an insurance company after Katrina.

After two hurricanes in three years, 74-year-old Tony Vegeletta vows he won't take a chance on another.

"I've had enough of this," said a disgusted Vegeletta as he cleaned Hurricane Gustav mud and water out of the ground-level garage of the house he built on stilts after Hurricane Katrina struck in 2005. "As soon as I sell this ... I'm gone."

___

BIRMINGHAM, Ala. (AP) _ Storm evacuees taking shelter in gymnasiums and convention centers across the south are a thankful lot. They're safe and, for the most part, well fed.

But life as a hurricane refugee can be stressful. James Katicich, 49, said his stay at a Birmingham shelter has been rough _ rationed portions of food and sleeping close to strangers.

"It seems like when you get on the other end, people don't care," he said. "They don't look at me like I'm a human. They treat me like a number."

He called it a "miserable 18-hour bus ride" from New Orleans to Birmingham because of evacuee traffic.

"But it's better to be safe than sorry," he said.

Friends who stayed behind in Louisiana called Chad Dykes, 26, to tell him his trailer in Livingston, La., had flipped over. But he still wishes he had stayed behind. Staying at the convention center shelter was too uncomfortable.

"I hate this," said Dykes, who drove with his parents in their truck to Birmingham. "I don't like being here. I can't sleep. I have no privacy."

Donald Carriere, 56 and a Red Cross volunteer, set up tables to serve lunch. He lived in New Orleans until he lost everything to Katrina. He now lives in Birmingham, and said he decided to volunteer because of his painful experience in 2005.

"Some of these people say they wish they had stayed in New Orleans since the storm was not that bad," Carriere said. "But I tell them it is always best to leave. Gustav could have been worse."

___

CHAUVIN, La. (AP) _ Few present the image of a stout, resolute Louisianan better than Darrell Domangue.

A boat captain who trawls for shrimp and lays crab traps, Domangue lives in the village of Chauvin, where a number of homes on pilings took on water in an area where the wind-driven surge appeared to approach 9 feet.

One of those homes was Domangue's century-old fishing camp. He was one of the only people in town on Tuesday, when he and a friend stood in knee-high water raking grass from under the house to keep snakes away.

His house sits on stilts about 5 feet above ground. He had at least 3 feet of water inside the house and now had to clean out about 5 inches of muck and ruined furniture.

"Ain't nothing we can't pick up and start again," he said.

Domangue's house was built by his grandfather. He lives there with his wife and his two youngest daughters, who went north to the central Louisiana city of Alexandria during the storm.

But Domangue never flees from storms. During Rita three years ago, the water in his camp was up to his neck, while papers and family photos kept atop a refrigerator washed away. This time, personal and sentimental items were taken by his wife, while Domangue _ sporting a tattoo of Moses on his biceps _ was determined to stay and clean up as soon as the winds died and the water receded.

"If you got good sense and faith you're going to make it. You can't go wrong. I got faith in God and I don't ever give up," he said. "I ain't going nowhere. I sent my wife and kids out of here, but the devil got to fight a lot harder to win me. If dad do bad, they all do bad, so dad can't give up."

___

MINDEN, La. (AP) _ National Guard troops from the Show-Me State are showing big support for coastal residents reeling from Gustav. But the cavalry can only go so fast.

Members of the 1139th Military Police Company in Missouri didn't seem daunted during their journey by the hours spent in hot, cramped Humvees and military trucks, nor by the uncertainty of what lay ahead.

"We moan and complain a lot during this part, but when we get there, we get out our gear and get to work," said Sgt. John Randall, 45.

The company made the journey at convoy speed, traveling about 50 mph in non-airconditioned Humvees. It means taking about four and a half hours to drive what a passenger car _ in cool comfort, mind you _ could cover in three hours.

But there were spirit-lifting stops for food and fuel at service stations along the way. The sight of two dozen Humvees and military trucks lined up at a Phillips 66 while uniformed troops milled around drew a lot of high-fives, waves and smiles.

"You have to wave a lot," Randall said.

___

Associated Press writers Holbrook Mohr in Pearlington, Miss., Juanita Cousins in Birmingham, Ala., Vicki Smith in Chauvin, La., and Maria Sudekum Fisher in Minden, La., contributed to this report.

UN and Islamic bank make $1 billion farming deal

A U.N. agency says a deal has been reached with the Islamic Development Bank for $1 billion in funding to help develop agriculture in poor countries.

The announcement comes on the eve of a U.N. summit in Rome to fight hunger worldwide.

The three-day summit starting Monday promotes an approach that U.N. officials say is vital to slashing the number of hungry people. The strategy envisions helping farmers in poor countries to grow enough food to feed their own people and reduce developing countries' reliance on food aid.

One goal proposed for the summit at the headquarters of the U.N. Food and Agriculture Organization is eradicating hunger by 2025, but a draft declaration does not include that goal or specify pledges for agricultural aid.

Moderate quake hits eastern Cuba

A magnitude-5.4 earthquake hit eastern Cuba early Friday, rattling nerves but causing no reported injuries or damage.

The temblor hit just after 7 a.m. Friday, centered about 35 miles (55 kilometers) southeast of Baracoa, near the easternmost tip of the island, according to the U.S. Geological Survey. That is just 160 miles (255 kilometers) from Port-au-Prince, Haiti, where a Jan. 12 quake destroyed much of the city and killed countless thousands.

"Yes we felt it. We felt it strong," said Maira Legra, whose son runs a home offering lodging to tourists in the colonial beach-side city of Baracoa. "There was no problem. I was in bed because it was early, but I didn't get up."

Wednesday, March 7, 2012

NYC to Hire Experts in Sept. 11 Search

NEW YORK - The renewed search for human remains around the World Trade Center site is so massive, the city plans to hire several more forensics experts.

Up to 10 forensic anthropologists will join the effort to find remains of Sept. 11 victims, a project that could stretch well into next year, Deputy Mayor Ed Skyler said Wednesday.

"We will make sure we have the appropriate resources to do this job," said Skyler, who is overseeing the recovery. "The mayor's orders were very clear: 'Do what needs to be done.'"

Meanwhile, the families of Sept. 11 victims planned to rally at ground zero on Thursday to call for federal intervention, a greater expansion of the remains search and a more clearly defined organization of the effort.

"Hiring extra anthropologists is a recognition that they realize the job they have to do," said Charles Wolf, whose wife was killed on Sept. 11. "But doing the job and doing it right are two different matters. How are you going to manage this?"

The current search was prompted by the discovery last month of human bones in an abandoned manhole.

Some 200 pieces of bone and other remains have been found since the accidental discovery by utility workers doing routine labor along the western edge of the lower Manhattan site.

Officials have said the manhole had been paved over and forgotten when a service road was built there in the midst of excavating trade center rubble years ago.

After the discovery, city officials identified about 10 more manholes and pockets under the road and ordered them to be excavated immediately and sifted for remains.

The work involves tearing up parts of side streets, exploring the rooftops of selected buildings near the 16-acre site and excavating more manholes beyond the dozen the city is already exploring.

Some families have pushed for the city to bring in the Joint POW/MIA Accounting Command, a military forensic unit that specializes in finding soldiers who went missing long ago.

---

Associated Press Writer Amy Westfeldt contributed to this report.

NYC to Hire Experts in Sept. 11 Search

NEW YORK - The renewed search for human remains around the World Trade Center site is so massive, the city plans to hire several more forensics experts.

Up to 10 forensic anthropologists will join the effort to find remains of Sept. 11 victims, a project that could stretch well into next year, Deputy Mayor Ed Skyler said Wednesday.

"We will make sure we have the appropriate resources to do this job," said Skyler, who is overseeing the recovery. "The mayor's orders were very clear: 'Do what needs to be done.'"

Meanwhile, the families of Sept. 11 victims planned to rally at ground zero on Thursday to call for federal intervention, a greater expansion of the remains search and a more clearly defined organization of the effort.

"Hiring extra anthropologists is a recognition that they realize the job they have to do," said Charles Wolf, whose wife was killed on Sept. 11. "But doing the job and doing it right are two different matters. How are you going to manage this?"

The current search was prompted by the discovery last month of human bones in an abandoned manhole.

Some 200 pieces of bone and other remains have been found since the accidental discovery by utility workers doing routine labor along the western edge of the lower Manhattan site.

Officials have said the manhole had been paved over and forgotten when a service road was built there in the midst of excavating trade center rubble years ago.

After the discovery, city officials identified about 10 more manholes and pockets under the road and ordered them to be excavated immediately and sifted for remains.

The work involves tearing up parts of side streets, exploring the rooftops of selected buildings near the 16-acre site and excavating more manholes beyond the dozen the city is already exploring.

Some families have pushed for the city to bring in the Joint POW/MIA Accounting Command, a military forensic unit that specializes in finding soldiers who went missing long ago.

---

Associated Press Writer Amy Westfeldt contributed to this report.

Our great green spaces ; Essex has some great country parks and seven of them have just been awarded Green Flag Awards

Seven Essex country parks have been awarded Green Flags, markingthem out as some of the best green spaces in the UK.

The country parks at Danbury, Weald and Thorndon near Brentwood,Great Notley near Braintree, Belhus Woods at South Ockendon,Hadleigh and Cudmore Grove at East Mersea will all proudly displayGreen Flags showing that they are well maintained and managed andhave excellent facilities.

A record number of Green Flag awards have been awarded this year,showing how many great green spaces there are in the country.

Standards "The Green Flag Award Scheme, and its growth, isessential in driving up the standards of our parks and greenspaces," said …

Putin urges for economy's diversification

MOSCOW (AP) — Prime Minister Vladimir Putin, who is running for the Russian presidency, says Russia must diversify its economy away from oil and gas to high-tech products to ensure its "sovereignty."

Putin wrote in his article in the Vedomosti daily on Monday that "Russia cannot afford to have an …

Tuesday, March 6, 2012

Wright gives it his best long shot.(Sports)

Byline: ALAN ROBINSON

PITTSBURGH -- BY ALAN ROBINSON

ASSOCIATED PRESS

PITTSBURGH - The Philadelphia Phillies haven't been big winners for a long time. Thanks to Ryan Howard and Bobby Abreu, they're tough to beat in the Home Run Derby.

Howard made certain his first trip to the All-Star Game would be a memorable one, beating out the Mets' David Wright to give the Phillies their second derby winner in as many summers.

"That'll give the fans in Philly something to cheer about," said Howard, whose 28 homers at the All-Star break tie him for second in the NL.

After homering into the Allegheny River earlier, the 2005 NL Rookie of the Year's fifth and decisive homer in the championship round banged off a "Hit It Here" …

Wright gives it his best long shot.(Sports)

Byline: ALAN ROBINSON

PITTSBURGH -- BY ALAN ROBINSON

ASSOCIATED PRESS

PITTSBURGH - The Philadelphia Phillies haven't been big winners for a long time. Thanks to Ryan Howard and Bobby Abreu, they're tough to beat in the Home Run Derby.

Howard made certain his first trip to the All-Star Game would be a memorable one, beating out the Mets' David Wright to give the Phillies their second derby winner in as many summers.

"That'll give the fans in Philly something to cheer about," said Howard, whose 28 homers at the All-Star break tie him for second in the NL.

After homering into the Allegheny River earlier, the 2005 NL Rookie of the Year's fifth and decisive homer in the championship round banged off a "Hit It Here" …

Wright gives it his best long shot.(Sports)

Byline: ALAN ROBINSON

PITTSBURGH -- BY ALAN ROBINSON

ASSOCIATED PRESS

PITTSBURGH - The Philadelphia Phillies haven't been big winners for a long time. Thanks to Ryan Howard and Bobby Abreu, they're tough to beat in the Home Run Derby.

Howard made certain his first trip to the All-Star Game would be a memorable one, beating out the Mets' David Wright to give the Phillies their second derby winner in as many summers.

"That'll give the fans in Philly something to cheer about," said Howard, whose 28 homers at the All-Star break tie him for second in the NL.

After homering into the Allegheny River earlier, the 2005 NL Rookie of the Year's fifth and decisive homer in the championship round banged off a "Hit It Here" …

Wright gives it his best long shot.(Sports)

Byline: ALAN ROBINSON

PITTSBURGH -- BY ALAN ROBINSON

ASSOCIATED PRESS

PITTSBURGH - The Philadelphia Phillies haven't been big winners for a long time. Thanks to Ryan Howard and Bobby Abreu, they're tough to beat in the Home Run Derby.

Howard made certain his first trip to the All-Star Game would be a memorable one, beating out the Mets' David Wright to give the Phillies their second derby winner in as many summers.

"That'll give the fans in Philly something to cheer about," said Howard, whose 28 homers at the All-Star break tie him for second in the NL.

After homering into the Allegheny River earlier, the 2005 NL Rookie of the Year's fifth and decisive homer in the championship round banged off a "Hit It Here" …

Monday, March 5, 2012

Partners HealthCare supervisor Jessica Gayden 'pays it forward'

Two years ago, Jessica Gayden felt like her professional life was going nowhere. Today, she's a hospital supervisor, helping others start careers in health care.

Gayden got her start through the Partners Career and Workforce Development Program (PCWD), a three-year old initiative started by Partners HealthCare that has helped nearly 600 Boston residents get entry-level jobs or advance up the career ladder at Brigham and Women's Hospital (BWH), Massachusetts General Hospital (MGH) and Spaulding Rehabilitation Hospital (SRH).

After hearing about PCWD from a family member, Gayden completed the five-week job readiness training program in September 2005. Two months later, she …

Challenges associated with the targeted delivery of gelonin to claudin-expressing cancer cells with the use of activatable cell penetrating peptides to enhance potency.(Research article)(Report)

Authors: Xiaoqin Yuan (equal contributor) [1]; Xinjian Lin (equal contributor) [1]; Gerald Manorek [1]; Stephen B Howell (corresponding author) [1]

Background

The claudin (CLDN) family of transmembrane proteins plays an integral role in the formation and function of tight junctions. Using gene expression profiling, we and others have found that claudin-3 (CLDN3) and claudin-4 (CLDN4) genes are highly expressed in ovarian cancers [1, 2, 3]. In addition, several other studies have reported aberrant claudin expression in various cancers. Some examples include increased expression of CLDN3 and CLDN4 in prostate and uterine cancers [4, 5], and high CLDN4 expression in pancreatic cancer [6, 7]. These two genes are not normally highly expressed in non-malignant human tissues including the normal ovarian epithelium [8], clearly associating abundance of these two proteins with malignancy. Although their functional role in cancer development and progression remains unclear, the differential expression of these proteins between tumor and normal cells makes them prime candidates for cancer targeted therapy [9]. Preclinical studies have shown that tumor cells over-expressing CLDNs can be successfully targeted both in vitro and in vivo by a fusion protein composed of the C-terminal fragment (amino acids 184 to 319) of Clostridium perfringens enterotoxin (CPE), a natural ligand for CLDNs, and the protein synthesis inhibitory factor (PSIF) which lacks the cell binding domain of Pseudomonas exotoxin [10, 11]. When CPE binds to CLDNs it triggers endocytosis most likely via a clathrin-dependent process. We previously reported in vitro characterization of a fusion protein, CPE[sub.290-319]-TNF, and demonstrated that the C-terminal 30 amino acids (amino acids 290-319) of CPE could effectively target TNF to ovarian cancer cells expressing claudin-3 and claudin-4 [12].

Gelonin (rGel) is a class I ribosome-inactivating protein derived from the plant Gelonium multiforum. Similar in action to other plant toxins such as ricin, gelonin induces cell death by removing the base A4324 in 28 s rRNA which prevents the association of elongation factor-1 and -2 (EF-1 and EF-2) with the 60 s ribosomal subunit, eventually causing cell death in eukaryotic cells [13]. Since gelonin functions enzymatically, only a few molecules are needed to kill a cell, but by itself gelonin has very limited toxicity because it is not able to cross the plasma membrane at levels that are therapeutically useful. This has prompted the development of strategies to improve intracellular accumulation. Gelonin has been used to construct a large number of different kinds of immunotoxins, some of which are currently undergoing clinical testing [14, 15, 16].

Cancer therapies that exploit targeting ligands to deliver attached cytotoxic drugs selectively to malignant cells are currently receiving significant attention. However, the lipophilic nature of the biological membranes restricts the direct intracellular delivery of such compounds. While some short peptides can enter cells, the cell membrane prevents large molecules, such as proteins and DNA, from entering cells unless there is an active transport mechanism. Under certain circumstances these molecules, or even small particles, can be transferred from the extracellular space into cells by the receptor-mediated endocytosis. However, the problem is that most molecules or particles entering the cell via the endocytic pathway become entrapped in endosomes and eventually get degraded in the lysosomal compartment. As a result, only a small fraction of active material reaches the cytoplasm. It has been reported that a poly-arginine tract such as R[sub.9], which is also a furin cleavage site, can aid in translocating a recombinant pro-apoptotic protein targeting the HER2 receptor from the endosomal to the cytosolic compartment leading to enhanced cell killing activity [17]. However, due to the fact that all the arginine-rich cell-penetrating peptides (CPPs) induce a strong non-specific cell binding, they lack cell specificity and this remains the major impediment to development. Tsien and coworkers [18] previously developed a new strategy, designated "activatable cell penetrating peptides (ACPP)" by which the cellular association of the positively charged R[sub.9 ]is effectively blocked by fusing it to a domain made up of negatively charged glutamates (E[sub.9]) via a cleavable linker. Adsorption and cellular uptake of the CPP portion and its attached cargo are inhibited until the linker is cleaved by a tumor protease. In the present study, we describe the construction and characterization of several rGel-based chimeric toxins composed of different combinations of CPE[sub.290-319], R[sub.9 ]and E[sub.9]. We have used these to examine how these modules affect the internalization and cytotoxic activity when tested against CLDN-expressing ovarian cancerous cells.

Methods

Reagents

Tissue culture media were purchased from Life Technologies (Frederick, MD), pE-SUMOstar vector and SUMOstar protease 1 from LifeSensors, Inc (Malvern, PA), and metal-affinity resin Ni-NTA agarose from Qiagen (Valencia, CA). Rabbit anti-gelonin antibody was a gift from Dr. Michael G. Rosenblum (MD Anderson Cancer Center, Houston, TX). Texas red-labeled secondary antibodies against rabbit immunoglobulin were obtained from Jackson ImmunoResearch Laboratories, Inc (West Grove, PA).

Cells and cell culture

The human ovarian carcinoma cell line 2008 that expresses both CLDN3 and CLDN4 and its CLDN3 knockdown subline 2008-CLDN3KD-4.5 [12] were grown in RPMI 1640 supplemented with 5% fetal bovine serum. Cultures were maintained at 37[degrees]C in a humidified atmosphere of 5% CO[sub.2 ]and 95% air.

Plasmid construction

The gene encoding gelonin in the …

New UAlbany chief makes early impression.(Capital Region)

Byline: KENNETH AARON Staff Writer

ALBANY - Kermit L. Hall vowed not to issue any sweeping pronouncements about his vision for the University at Albany on Wednesday afternoon, his second day on the job.

But he still had his new constituents applauding at a ceremony marking his arrival by announcing a new scholarship fund, seeded with $110,000, and vowing to relocate the president's office from its isolated Western Avenue roost to the center of campus.

"The president is moving from Gilligan's Island over to the podium," Hall said to applause, referring to the cluster of academic buildings that make up the core of UAlbany.

For several days a …

Lack of funds for parking garage worries Redmond.

Byline: Jeff McDonald

May 26--Plans to revitalize downtown Redmond with as much as $90 million in redevelopment projects could collapse if the city cannot work out a deal to pay for a parking garage, according to City Manager Mike Patterson.

Without the garage, The ODS Cos. could nix its plans to build a $22 million office and dental-hygienist school downtown with 120 employees.

It also could jeopardize a planned renovation of the historic Redmond Hotel.

"I think people need to realize that it is very possible for this (ODS) deal to fall apart," Patterson said. "The loss of this deal could really hurt downtown Redmond."

Loss of …

Inquiry begins into hacking scandal

LONDON (AP) — A senior judge on Thursday opened an inquiry into Britain's phone-hacking scandal that will start by looking at whether the country needs tougher media regulation.

Justice Brian Leveson said he has the legal power to demand evidence from witnesses — and plans to use it "as soon as possible."

Leveson's inquiry was announced earlier this month by Prime Minister David Cameron in the wake of a scandal over illegal eavesdropping that has closed down the 168-year-old News of the World tabloid and shaken Rupert Murdoch's global media empire.

Leveson's 7-member panel includes a veteran newspaper reporter, a former police chief, a civil liberties activist and a …

Who nose how mammals smell? Scientist says he does

NEW YORK -- By studying blindfolded college students who crawledthrough grass to sniff out a chocolate-scented trail, scientists saythey've found evidence of a human smelling ability that expertsthought was impossible.

The study indicates the human brain compares information it getsfrom each nostril to get clues about where a smell is coming from.And it suggests dogs, mice and other mammals do the same thing,contrary to what most scientists have thought.

People compare signals from each ear to locate the source of anoise.

But the prevailing notion has been that mammals can't follow thesame strategy for smells, because their nostrils are too closetogether …

Sunday, March 4, 2012

After Denufosol Failure, Inspire Ripe to Buy or Be Bought.

Staff Writer

Shares of Inspire Pharmaceuticals Inc. fell 58.7 percent Monday after the firm said cystic fibrosis drug denufosol failed to meet its primary and secondary endpoints in a Phase III trial.

The setback was "both disappointing and unexpected," Inspire President and CEO Adrian Adams said during a conference call u particularly because a previous, similarly designed Phase III trial had succeeded.

The first trial u dubbed TIGER-1 u met its primary endpoint of improved forced expiratory volume in one second (FEV1), with a 45 mL difference between the denufosol-treated group and the placebo group after 24 weeks (p = 0.047). FEV1 continued to improve in a 24-week extension study. …

Robbery at convenience store.

A Bury St Edmunds man has been arrested on suspicion of robbery last night.

A robbery took place at A & B General …

SHUTTLE AMERICA ADDS ALBANY.(BUSINESS)

Byline: LARA JAKES Capitol bureau

The first flight between Albany and Buffalo had a record-breaking 39 passengers on board; the second, only 12. But that's not counting the pack of special guests who climbed into 50-seat Shuttle America plane Tuesday morning for a quick inspection of the low-price carrier connecting Albany to five other Northeast destinations.

``You going to see the plane?'' quipped Gov. George Pataki, loping toward the tarmac where the $10 million craft, headed to Buffalo, awaited its passengers on its first day of cross-state service. He deplaned before the shuttle took to the skies, but not before checking out the sleek craft.

JohnsonDiversey Shifts to Distributors.

JohnsonDiversey says it will begin to sell through distributors within its U.S. health care and hospitality business, a shift from its company-owned sales force model. JohnsonDiversey is not exiting the U.S. health care and hospitality business, but it is changing its business model to a "low-to-no- service required" …

Spanish Football Standings

GP W D L GF GA Pts
Barcelona 11 9 1 1 36 8 28
Real Madrid 12 8 2 2 29 17 26
Villarreal 12 7 4 1 21 14 25
Valencia 12 7 3 2 25 12 24
Sevilla 12 7 3 2 18 8 24
Atletico Madrid 11 5 2 4 23 17 17
Valladolid 12 5 1 6 13 17 16
Deportivo La Coruna 11 4 3 4 10 13 15

Cozying Up to the Customer ; How a once-struggling credit union is using software to entice more business from its members.

In the mid-1990s, a sleepy IBM employees' credit union woke up to the fact that it needed to market itself more aggressively. Today, the changes are quite visible for visitors to Meriwest Credit Union's branches in River Oaks and Milpitas, Calif., where customers can sip Peet's Coffee in a cybercafe that features access to online banking services. These updated branches, with their granite countertops and slate floors, are purposefully designed to dramatize the changes in the former Pacific IBM Credit Union.

But the changes at Meriwest run deeper than interior decorating, and include a customer relationship management (CRM) architecture that connects Siebel eFinance software …